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TOXICOLOGY

Elisa

Enzyme-Linked Immunosorbent Assay (ELISA) screening techniques are widely utilized by toxicologists to screen forensic specimens for drugs of abuse. These immunoassays are flexible and have adequate sensitivity to detect the low drug levels found in most forensic matrices.

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ELISA
Benefits

ADVANTAGES OF ELISA
 

  • Heterogeneous assays with oriented antibodies and optimized enzyme conjugates maximize sensitivity
  • Ready to use reagents, minimal sample pretreatment and automation options improve your lab's workflow efficiency
  • Low sample volume requirements with a broad variety of forensic matrices for expanded functionality
  • Long shelf life (minimum one year) 
  • No azide or mercury preservatives 
PRINCIPLE OF ELISA
Elisa infographic Elisa infographic Elisa infographic
  • Antibody is immobilized on microplate well 
  • Competition between drug in forensic sample and enzyme-labeled drug for antibody binding sites 
  • Unbound material is washed out 
  • Chromogenic substrate added to develop color with bound enzyme 
  • Resulting color is read in a spectrophotometer
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